MOLEQULE-ON, New Zealand
25X TAE (Tris-Acetate-EDTA) Buffer
25X TAE (Tris-Acetate-EDTA) Buffer
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25X TAE (Tris‑Acetate‑EDTA) Buffer — concentrated liquid stock for molecular biology applications; intended to be diluted to 1X for use in agarose gel preparation and electrophoretic runs. Manufactured by MOLEQULE‑ON, Auckland, New Zealand; supplied by EMDA. (Size: 1 L)
Key specifications & preparation: Dilute 25X stock 1:25 to obtain 1X TAE (approximately 40 mM Tris‑acetate, 1 mM EDTA, pH ≈ 8.3), which is used as both gel and running buffer.
Applications & benefits: Recommended for agarose gel electrophoresis of nucleic acids (including genomic and large/supercoiled DNA) and for resolving larger fragments (commonly >1,500 bp). Suitable as both gel preparation buffer and running buffer in standard molecular biology workflows.
Performance notes & handling: TAE has lower buffering capacity than some alternatives; for extended runs consider buffer circulation or replacement. For best resolution keep applied voltages moderate (Sigma recommends values below ~5 V/cm to limit overheating and maintain resolution).
Storage & quality: Supplied as a 25X concentrate in a 1 L bottle; store at room temperature and handle per standard laboratory safety and waste disposal practices.
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