Western Blocking Buffer (milk)

Western Blocking Buffer (milk) — an improved, milk-based blocking solution for Western blotting. Manufactured by MOLEQULE-ON, New Zealand, and supplied by EMDA, this ready-to-use liquid uses non-fat milk proteins to reduce nonspecific antibody binding and lower background while preserving target signal.

• Specifications: 500 ml ready-to-use liquid; store refrigerated at 2-8°C; consult the product label and SDS for shelf life and safety information.
• Key features: reduces background and nonspecific binding; can be used as an antibody diluent; offers consistent performance versus homemade milk preparations; easy-to-use ready solution that saves preparation time.
• Applications & compatibility: optimized for blocking nitrocellulose (NC) and PVDF membranes in Western blot workflows; compatible with chemiluminescent and colorimetric detection methods. Not recommended for avidin-biotin detection systems or when probing phospho-epitopes that may be masked by milk components.
• Usage notes: follow laboratory-validated blocking times and antibody dilutions (typical blocking 30-60 minutes at room temperature or as optimized); adjust detergent in wash buffers as needed; refer to label for handling, disposal, and any assay-specific recommendations.