RPMI 1640 Medium

RPMI 1640 Medium — RPMI 1640 Medium is the classical Roswell Park formulation for mammalian cell culture and remains widely used for lymphoid, hybridoma, and many adherent and suspension cell lines. (en.wikipedia.org)

Manufacturer: MOLEQULE-ON, New Zealand; Supplier: EMDA; Size: 500 mL.

Overview & key specifications: Balanced salt and nutrient formulation containing glucose, amino acids, and a rich complement of vitamins (including biotin, vitamin B12, and PABA) and the reducing agent glutathione; uses a bicarbonate buffering system and is formulated to be used in a 5–10% CO2 atmosphere to maintain physiological pH (~7.2–7.4). Typical osmolarity and composition enable robust growth of many mammalian cell types. (thermofisher.cn)

Typical use & recommended supplements: Commonly supplemented with fetal bovine serum (FBS, often 5–10%) and L-glutamine when required by the cell line; suitable for culturing PBMCs, lymphoid cell lines, hybridomas, established cancer cell lines, and many primary mammalian cells for applications such as proliferation assays, immunology, transfection, and viral assays. (thermofisher.cn)

Handling & storage: Store as directed by the supplier (commonly 2–8°C for liquid formulations); equilibrate in incubator CO2 before use, inspect lot-specific certificate of analysis (COA) for sterility and performance data. (sigmaaldrich.com)

Notes: This description enhances the existing product name and summarizes formulation highlights, manufacturer (MOLEQULE-ON, New Zealand), and supplier (EMDA). For exact formulation details, sterility, expiration, and lot-specific QC, consult the supplier documentation or COA prior to use.