Solis Biodyne Ou
PCR Grade Water (25 mL)
PCR Grade Water (25 mL)
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Product Overview
Manufacturer: Solis BioDyne
Catalog Number: WATER-025
Size: 25 mL
Description: Solis BioDyne's PCR Grade Water is ultrapure, deionized, and autoclaved water, free from DNase, RNase, and protease activities. It is prepared without chemical additives, ensuring suitability for all experiments requiring nuclease-free water. This product is ideal for PCR, qPCR, RT-qPCR, and other molecular biology applications where pure water is essential. Storage at -20°C is recommended to maintain full activity until the expiry date printed on the tube label. Shipping and temporary storage for up to 1 month at room temperature (15–25°C) have no detrimental effects on the quality of Solis BioDyne reagents. Freeze-thaw stability is tested for each product, with most PCR and qPCR products passing 30 freeze-thaw cycles with no changes in performance. Specific information is found in the Storage and Shipping conditions of each product. (solisbiodyne.com)
Specifications:
- Component: PCR Grade Water
- Volume: 25 mL
- Storage Conditions: On arrival, products can be stored at room temperature. Once opened, care should be taken to avoid contamination, and storage between -30 and -20°C is recommended. The product can be stored indefinitely prior to first use. (pcrbio.com)
Applications:
- PCR
- qPCR
- All molecular biology reactions
- Sample dilution
- Use as no-template control
Quality Control:
- RNase Activity: Absence of RNase activity was checked by incubation of RNA in PCR Grade Water at 37°C for 3 hours. No degradation detected.
- DNase Activity: PCR Grade Water was incubated with DNA fragments and Plasmid DNA for 14–16 hours at 37°C. Samples were checked on an agarose gel. No degradation detected.
- Protease Activity: A reference protein was incubated in PCR Grade Water for 3 days at 37°C. Samples were checked on a polyacrylamide gel. No degradation detected.
- Bioburden: The number of viable aerobic microorganisms present in PCR Grade Water was tested using membrane filtration and incubation for 3–5 days. No growth detected. (biotechrabbit.com)