HotStarTaq Plus DNA Polymerase (1000)

Product Overview

HotStarTaq Plus DNA Polymerase is a recombinant enzyme designed for fast and highly specific amplification in various PCR applications. It offers a rapid 5-minute activation time at 95°C, ensuring minimal nonspecific amplification. The enzyme is supplied in an inactive state at ambient temperatures, preventing extension of nonspecifically annealed primers and primer dimers during PCR setup. Activation is achieved through a brief 5-minute incubation at 95°C, seamlessly integrating into existing thermal-cycler programs.

Specifications

• Concentration: 5 units/µl
• Extension Rate: 2–4 kb/min at 72°C
• Half-Life: 10 min at 97°C; 60 min at 94°C
• 5'–3' Exonuclease Activity: Yes
• Extra A Addition: Yes
• 3'–5' Exonuclease Activity: No
• Contaminating Nucleases: No
• Contaminating RNases: No
• Contaminating Proteases: No
• Self-Priming Activity: No

Manufacturer Information

Manufacturer: Qiagen GmbH
Material Number: 203605
Material Description: HotStarTaq Plus DNA Polymerase (1000)

Storage and Handling

Store at ?20°C. Avoid repeated freeze-thaw cycles to maintain enzyme activity.

Applications

Ideal for PCR, RT-PCR, amplification of complex genomic templates, very low-copy targets, and reactions with multiple primer pairs.

Pricing

Price: $792.00 per 1000 units

Sources

• Qiagen Official Product Page
• Qiagen Product Details
• Fisher Scientific Product Listing
• Labsave Product Information
• QIAGEN 2022 Price List