HOT FIREPol¨ EvaGreen¨ HRM Mix (no ROX)

HOT FIREPol® EvaGreen® HRM Mix (no ROX)

2x HRM master mix containing Hot FIREPol polymerase and EvaGreen dye supplied without ROX — formulated for robust HRM results at moderate scale.

• Manufacturer: Solis BioDyne; Supplier: AFMS
• Pack size: 20.0 ml (5000 rxn)
• Applications: high-resolution melting, genotyping and mutation scanning
• Key features: hot-start formulation for high specificity, EvaGreen for strong signal with low inhibition; no ROX
• Format & use: 2x concentrate — mix equal volumes with template/primers to obtain 1x; compatible with HRM-capable cyclers that do not require ROX
• Storage: store at −20°C; aliquot to reduce freeze–thaw