Hi-Tech Green 2X qPCR Mix

Hi-Tech Green 2X qPCR Mix — Hi-Tech Green 2X qPCR Mix is a 2X concentrated, ready-to-use master mix formulated for dye-based (Hi-Tech Green I) quantitative PCR. Manufacturer: MOLEQULE-ON (New Zealand). Supplier: EMDA. Size: 1 ml.

Key features:

• Contains a NanoTaq hot-start DNA polymerase, balanced dNTPs, MgCl2, proprietary enhancers and Hi-Tech Green I dye for high sensitivity and specificity in SYBR-style assays.
• Optimized to reduce nonspecific products and compatible with most real-time PCR instruments (ROX-dependent and ROX-independent platforms).
• Supplied as a convenient 2X master mix for simple 1X final reaction setup.

Typical applications:

• Gene expression analysis (absolute and relative quantification)
• Detection of low-copy targets and viral load estimation
• Quantification of NGS libraries and routine dye-based qPCR workflows

Performance & recommendations: For best results use the mix at 1X final concentration in standard qPCR reaction volumes and design amplicons in the 80-200 bp range for optimal amplification and melting-curve analysis. Follow the manufacturer's cycling and handling instructions to preserve enzyme activity and assay performance.