DMEM High-Glucose, 500ml

DMEM High-Glucose, 500ml — Dulbecco's Modified Eagle Medium (high-glucose) provided in a 500 ml sterile liquid format; enhanced here with key specifications, manufacturer and practical use guidance to supplement the existing product title.

Manufacturer: MOLEQULE-ON (New Zealand). Supplier: EMDA.

Overview: Standard high-glucose DMEM contains approximately 4.5 g/L (4500 mg/L) glucose and is formulated to support robust growth and metabolism of a broad range of mammalian cell lines. (sigmaaldrich.com)

• Typical formulation notes: many high-glucose DMEMs include L-glutamine and phenol red; some variants also contain sodium pyruvate or HEPES — check the product label for the exact additive set supplied with this bottle. (sigmaaldrich.com)
• Common supplements: routinely supplemented with serum (e.g., 10% FBS) and optionally antibiotics; concentrations and supplements depend on the cell type and application. (fishersci.com)
• Applications / cell types: widely used for adherent and transformed mammalian cells such as HEK293/293T, HeLa, primary fibroblasts and many neuronal and epithelial cultures. Ideal for routine maintenance, expansion and many transfection workflows. (fishersci.com)
• Incubation & buffering: DMEM uses a bicarbonate/CO2 buffering system and is normally used in a 5% CO2 incubator (adjust CO2 to match the medium's bicarbonate level when required). (thermofisher.com)
• Handling & storage: supplied sterile-filtered; store unopened at 2-8°C and avoid repeated freeze-thaw or prolonged exposure to room air to preserve pH and sterility. Verify lot COA and expiration on the bottle before use. (sigmaaldrich.com)

Practical tips: pre-warm to 37°C before use, aseptically supplement per your lab protocol, and monitor pH/color as an early indicator of contamination or metabolic stress. For critical or specialized applications (serum-free, low-glucose metabolism studies, stem cell culture) select the exact DMEM variant or a defined medium appropriate to the protocol.