Absolute Master Mix (2X Ready Mix for conventional PCR)

Absolute Master Mix (2X Ready Mix for conventional PCR)

Absolute Master Mix (2X Ready Mix for conventional PCR) — an enhanced ready-to-use 2X PCR master mix that builds on the existing formulation: contains recombinant thermostable DNA polymerase, optimized PCR buffer with Mg²+, dNTPs and loading dyes; requires only addition of primers, template DNA and nuclease-free water. Manufacturer: MOLEQULE-ON, New Zealand. Supplied volume: 1.25 ml.

(molequle-on.com)

• Key features
  • 2X formulation for simple 1X reaction setup (just add primers, template and water)
  • Fast extension capability (example: completes ~2 kb in ≈60 seconds under optimized conditions)
  • High sensitivity for low-copy templates and proven amplification of long targets (up to ≈5 kb from lambda DNA)
  • Contains loading dyes to allow direct gel loading of PCR products, saving time in post-PCR processing
  • Robust stability profile reported by the manufacturer (stable for 21 days at 37°C under tested conditions)

(molequle-on.com)

Suggested use & handling: prepare reactions at 1X final concentration, thaw mix on ice, pipette gently, briefly centrifuge and avoid repeated freeze–thaw cycles. Store long-term at -20°C and keep on ice during setup for best performance.

(neb.com)

Applications: routine conventional PCR, colony PCR, amplification from plasmid or genomic templates where a rapid, sensitive and cost-effective 2X master mix is required.