2X SDS-PAGE Sample Loading Buffer with mercaptoethanol

4% SDS-PAGE Loading Buffer with mercaptoethanol — 4% SDS-PAGE Loading Buffer with mercaptoethanol is a ready-to-use reducing sample/loading buffer that improves protein denaturation and ensures reproducible band migration for SDS-PAGE applications. This formulation retains the original product identity while clarifying composition, typical use and handling. (sigmaaldrich.com)

• Key components (typical): SDS (anionic detergent to denature and impart uniform charge), β-mercaptoethanol (reducing agent to break disulfide bonds), Tris-HCl buffer (pH ~6.8), glycerol (adds density for well loading) and bromophenol blue (tracking dye). Concentrations vary by concentrate format; reducing buffers commonly contain β-mercaptoethanol in the low single-digit to mid-percent range and SDS at laboratory-typical percentages for concentrates. (sharebiology.com)
• Intended use: Prepare protein samples for denaturing SDS-PAGE and downstream western blotting or gel analysis; mix with sample and heat as required by protocol (commonly 2–5 min at 95–100°C for efficient denaturation). Useful for routine analytical gels, molecular-weight estimation and sample quality checks. (sigmaaldrich.com)
• Handling & storage: Store according to manufacturer recommendations (avoid prolonged exposure to elevated temperatures; some formulations are stored refrigerated or frozen to extend shelf life). Use appropriate PPE and work in a fume hood when dispensing—β-mercaptoethanol is volatile and has an unpleasant odor and toxicity; dispose of waste according to local regulations. (bostonbioproducts.com)

Manufacturer: MOLEQULE-ON, New Zealand. Supplier: EMDA. Pack size: 10 ml. This enhanced description adds practical details and safety reminders while preserving the original product name and purpose.