10X TE (Tris-EDTA) Buffer, pH 7.4

5X TE (Tris-EDTA) Buffer, pH 7.4 — concentrated Tris/EDTA buffer for routine molecular biology. Manufacturer: MOLEQULE-ON (New Zealand). Supplier: EMDA. Pack size: 500 mL.

Key specifications

• Formulation (5X): 50 mM Tris-HCl and 5 mM EDTA (final 1X = 10 mM Tris-HCl, 1 mM EDTA); pH 7.4.
• Concentrated ready for preparation of 1X working solution (dilute 1:5 with nuclease-free water).
• Pack volume: 500 mL (as supplied).

Primary features & benefits

• EDTA chelates divalent cations (Mg2+/Ca2+), helping inhibit DNases/RNases and stabilise nucleic acids during storage and handling.
• Low ionic-strength Tris buffering maintains pH for downstream enzymatic reactions and nucleic acid integrity.
• Convenient concentrated format reduces shipping volume and storage space.

Typical applications: long-term DNA/RNA storage, resuspension of plasmid or genomic DNA, preparation of PCR templates and sequencing samples, gel-extraction and cleanup workflows, and as a component of transformation/lysis buffers in molecular protocols.